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345797

Sigma-Aldrich

α1-3,6-Galactosidase, Recombinant, E. coli

Catalyzes the hydrolysis of α1,3-linked and α1,6-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.

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Synonym(s):
α1-3,6-Galactosidase, Recombinant, E. coli, α1-3,6-D-Galactoside Galactohydrolase
CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

liquid

specific activity

≥30 units/mg protein
≥85 units/mL

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

Proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

General description

Recombinant α1-3,6-galactosidase expressed in E. coli. Catalyzes the hydrolysis of α1,3-linked and α1,6-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. Particularly efficient in removing α-linked galactose under conditions where the pH must be neutral or above, as with live cells.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-α-D-galactopyranoside per min at 25°C, pH 6.5.

Physical form

In 50 mM sodium phosphate buffer, pH 7.5.

Other Notes

Prime, S., et al. 1996. J. Chromatogr. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2


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