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345795

Sigma-Aldrich

β1,3-Galactosidase, Xanthomonas manihotis, Recombinant, E. coli

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Synonym(s):
β1,3-Galactosidase, Xanthomonas manihotis, Recombinant, E. coli, β-D-Galactoside Galactohydrolase
CAS Number:
9031-11-2
Enzyme Commission number:
3.2.1.23 (BRENDA, IUBMB)
UNSPSC Code:
12352202

recombinant

expressed in E. coli

Quality Level

100

form

liquid

specific activity

≥2000 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

foreign activity

β-N-acetylglucosaminidase, α-fucosidase, α-galactosidase, β-glucosidase, α- and β-mannosidase, proteases, none detected

shipped in

wet ice

storage temp.

−20°C

General description

Recombinant, Xanthomonas manihotis β1,3-Galactosidase expressed in E. coli. Cleaves terminal β1,3-linked galactose residues on oligosaccharides. Has >100-fold kinetic preference for β1,3- over β1,6-linkages and 500-fold kinetic preference for β1,3- over β1,4-linkages.
Recombinant, Xanthomonas manihotis β1,3-Galactosidase expressed in E. coli. Cleaves terminal β1,3-linked galactose residues on oligosaccharides. Has >100-fold kinetic preference for β1,3-over β1,6-linkages and 500-fold kinetic preference for β1,3-over β1,4-linkages.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will cleave >95% of the β-D-galactose from 1 nmol Galβ1,3GlcNAcβ1,3Galβ1,4Glc-7-amino-4-methylcoumarin in 1 h at 37°C, pH 4.5 in a total volume of 10 µl.

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 100 µM EDTA, pH 7.5.

Other Notes

Taron, C., et al. 1995. Glycobiology 5, 603.
Wong-Madden, S.T., and Landry, D. 1995. Glycobiology 5, 19.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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