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344906

Sigma-Aldrich

FURA 2/AM in Solution

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Synonym(s):
FURA 2/AM in Solution
Empirical Formula (Hill Notation):
C44H47N3O24
CAS Number:
108964-32-5
Molecular Weight:
1001.85
MDL number:
MFCD00036976
UNSPSC Code:
12171500
NACRES:
NA.47

Assay

≥95% (HPLC)

Quality Level

100

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

fluorescence

λex 335 nm (high)
λex 362 nm (low)
λem 505 nm (high)
λem 512 nm (low)

shipped in

ambient

storage temp.

−20°C

InChI

1S/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3

InChI key

UTBYGIDPBQOHNT-UHFFFAOYSA-N

General description

Cell-permeable ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (Kd = 224 nM) that offers 30X greater fluorescence intensity that QUIN 2 (Cat. No. 551826). This probe has been used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm).

Biochem/physiol Actions

Cell permeable: no
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical form

A 1 mM solution of FURA 2/AM (Cat. No. 344905) in anhydrous DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Store frozen under an inert atmosphere.

Other Notes

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.
Schwartz, Y., et al. 1992. Endocrinology131, 772.
Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

closed cup

Flash Point(C)

closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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