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Merck
CN

34321-M

FITC, Isomer I

Synonym(s):

FITC, Isomer I, Fluorescein Isothiocyanate, Isomer I

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About This Item

Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
3326-32-7
Molecular Weight:
389.38
UNSPSC Code:
41116133
MDL number:
MFCD00005063
NACRES:
NA.41

Key Documents

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SMILES string

S=C=Nc1cc2c(cc1)C3(OC2=O)c4c(cc(cc4)O)Oc5c3ccc(c5)O

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

assay

≥95% (TLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

orange-red

solubility

DMSO: 5 mg/mL
DMF: soluble
aqueous buffer, pH ≥ 9.0: soluble

storage temp.

2-8°C

Quality Level

100

Preparation Note

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

General description

A fluorescent labeling agent used to label antibodies, lectins, toxins, various proteins, and oligopeptides with fluorescein. FITC-antibody conjugates are useful in many applications, including flow cytometry and immunohistochemistry (excitation maximum = 492 nm; emission maximum = 520 nm). λ max: 492 ± 3 nm (pH 9). Fluorescence EMmax: 520 ± 3 nm (pH 9).
Popular derivative of fluorescein. Can be used to label antibodies and other proteins.

Other Notes

Cherry, N.B., et al. 1965. Stain Technol. 44, 179.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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