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343209

Sigma-Aldrich

Fluorescein Diacetate

≥99% (HPLC), solid, esterase substrate, Calbiochem®

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Synonym(s):
Fluorescein Diacetate
Empirical Formula (Hill Notation):
C24H16O7
CAS Number:
Molecular Weight:
416.38
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

colorless to yellow

solubility

ethanol: soluble

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

InChI key

CHADEQDQBURGHL-UHFFFAOYSA-N

General description

Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.
Substrate for lipases and esterases. Abs. Max: 498 nm.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Unstable in solution. Reconstitute just prior to use.

Other Notes

Clements, K.M., et al. 2001. Osteoarthritis Cartilage9, 499.
Bedner, E., et al. 1998. Cytometry33, 1.
Guilbault, G.G., and Kramer, D.N. 1964. Anal. Chem. 36, 409.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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