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Sigma-Aldrich

Elastase Substrate V, Fluorogenic

≥95% (HPLC), powder, lyophilized solid, elastase substrate, Calbiochem®

Synonym(s):

Elastase Substrate V, Fluorogenic, MeOSuc-AAPV-AMC

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About This Item

Empirical Formula (Hill Notation):
C31H41N5O9
CAS Number:
72252-90-5
Molecular Weight:
627.69
MDL number:
MFCD00077135
UNSPSC Code:
12352200
NACRES:
NA.25

product name

Elastase Substrate V, Fluorogenic, Elastase Substrate V, Fluorogenic, CAS 72252-90-5, is a sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (Km = 140 µM).

Quality Level

200

Assay

≥95% (HPLC)
≥95% (TLC)

form

lyophilized solid
powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

white to off-white

solubility

ethanol: 1 mg/mL
DMF: 5 mg/mL
DMSO: 5 mg/mL

fluorescence

λex 360-380 nm
λem 430-460 nm

shipped in

ambient

storage temp.

−20°C

InChI

1S/C31H41N5O9/c1-16(2)27(34-20-9-10-21-17(3)14-26(39)45-23(21)15-20)30(42)35-29(41)22-8-7-13-36(22)31(43)19(5)33-28(40)18(4)32-24(37)11-12-25(38)44-6/h9-10,14-16,18-19,22,27,34H,7-8,11-13H2,1-6H3,(H,32,37)(H,33,40)(H,35,41,42)/t18-,19-,22-,27-/m0/s1

InChI key

NHMQNKPYBPDCAY-NFHWZJRKSA-N

General description

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (Km = 140 µM; Kcat/Km = 120,000 s-1 M-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (Km = 140 µM; kcat/Km = 120,000 M-1 s-1). Not hydrolysed by cathepsin G.

Biochem/physiol Actions

Cell permeable: no
Km = 140 µM; kcat/Km = 120,000 M⁻¹ s⁻¹as substrates for human leukocyte and porcine pancreatic elastase
Primary Target
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

MeOSuc-Ala-Ala-Pro-Val-AMC

Reconstitution

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.

Other Notes

Barrett, A.J. 1981. Methods Enzymol. 80, 581.
Castillo, M.J., et al. 1979. Anal. Biochem.99, 53.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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