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324717

Sigma-Aldrich

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

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Synonym(s):
Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli, Endo-β-N-acetylglucosaminidase H, Endo H
CAS Number:
52769-51-4
Enzyme Commission number:
3.2.1.96 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

100

conjugate

(N-linked)

form

liquid

specific activity

≥3 units/mL
≥30 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Note: 1 mU = 1 milliunit.
Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Other Notes

Rao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

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