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Merck
CN

324626

EGTA, Molecular Biology Grade

Synonym(s):

EGTA, Molecular Biology Grade, Ethyleneglycol- bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetic Acid

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About This Item

Empirical Formula (Hill Notation):
C14H24N2O10
CAS Number:
Molecular Weight:
380.35
UNSPSC Code:
12352106
NACRES:
NA.25
MDL number:
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SMILES string

N(CCOCCOCCN(CC(=O)O)CC(=O)O)(CC(=O)O)CC(=O)O

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

InChI key

DEFVIWRASFVYLL-UHFFFAOYSA-N

grade

Molecular Biology

description

RTECS - AH3760000

assay

≥98% dry basis (complexometric)

form

solid (fine)

reaction suitability

reagent type: chelator

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

1 M NaOH: soluble

cation traces

heavy metals (as Pb): ≤0.001%

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

10-30°C

Quality Level

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Application


  • Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.: This study presents a genetically encoded biosensor for real-time monitoring of lysosomal pH changes, potentially enhanced by buffering agents like EGTA. The findings may offer insights into cellular mechanisms influenced by calcium chelation (Ponsford et al., 2021).

  • Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca(2+).: The research highlights the role of calcium in fungal development, with EGTA potentially influencing these biological processes through calcium chelation (Zhu et al., 2019).


Disclaimer

Toxicity: Standard Handling (A)

General description

Chelating agent selective for Ca2+. Binds one cation per molecule. Induces apoptosis in pheochromocytoma (PC12) cells.

Other Notes

Lindenboim, L., et al. 1995. J. Neurochem.64, 1054.
Bers, D.M. 1982. Am. J. Physiol.242, C404.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 year at 4°C.

Storage Class

11 - Combustible Solids

wgk

WGK 2


Certificates of Analysis (COA)

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