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322327

Sigma-Aldrich

Diphtheria Toxin, CRM Mutant

Diphtheria Toxin, CRM197 Mutant, CAS 92092-36-9, is a nontoxic material with a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity.

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Synonym(s):
Diphtheria Toxin, CRM Mutant
CAS Number:
92092-36-9
UNSPSC Code:
12352200

Quality Level

100

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

ambient

storage temp.

2-8°C

General description

CRM197 is a nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity. Does not catalyze the ADP ribosylation of EF-2 factor. Immunologically, the CRM197 protein is identical to the wild-type toxin.
Cross-reactive material of diphtheria toxin (CRM197). A nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A that results in complete loss of enzymatic activity. Inhibits smooth muscle cell proliferation in a dose-dependent manner. Immunologically, the CRM197 is identical to the wild-type toxin and is useful as a carrier protein of conjugate vaccines.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Mutant with loss of enzymatic activity to catalyze the ADP ribosylation of EF-2 factor
Reversible: no

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.
Please refer to vial label for lot-specific reconstitution volume.

Analysis Note

One major band by SDS-PAGE

Other Notes

Kusano, I., et al. 2001. Cell Struct. Funct.26, 279.
Kaefer, M., et al. 2000. J. Urol.163, 580.
Gupta, R.K., et al. 1997. Vaccine15, 1341.
Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol.251, 175.
Giannini, G., et al. 1984. Nucleic Acids Res.12, 4063.
Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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