Skip to Content
Merck
CN
All Photos(1)

Documents

322123

Sigma-Aldrich

5,5′-Dithio-bis-(2-nitrobenzoic Acid)

Sulfhydryl reagent used to characterize reactive SH groups.

Synonym(s):

5,5′-Dithio-bis-(2-nitrobenzoic Acid), DTNB, Ellman’s Reagent

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C14H8N2O8S2
CAS Number:
69-78-3
Molecular Weight:
396.35
MDL number:
MFCD00007140
UNSPSC Code:
12352200
NACRES:
NA.21

Quality Level

100

Assay

≥98% (titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

yellow

solubility

ethanol: 8 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

Related Categories

General description

Sulfhydryl reagent used to characterize reacting SH groups. Useful in photometric determination of thiols.
Sulfhydryl reagent used to characterize reactive SH groups. Useful in photometric determination of thiols.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Useful in photometric determination of thiols
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Preparation Note

May require gentle heating for complete solubilization.

Other Notes

Ellman, G.L., et al. 1961. Biochem. Pharmacol.7, 88.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Protocols for the Fmoc SPPS of Cysteine-Containing Peptides

Overcome challenges in synthesis and disulfide bond formation with protocols for Fmoc solid-phase peptide synthesis of peptides with cysteine and methionine.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service