317640-M
Dipeptidyl Peptidase IV (CD26), Porcine Kidney
Synonym(s):
CD26, DPPIV
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Assay
≥90% (SDS-PAGE)
Quality Level
form
liquid
specific activity
≥35 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
storage temp.
−70°C
General description
Native, DPP IV purified from porcine kidney (renal brush border of the proximal tubule). A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. Cleaves Xaa-Pro dipeptides from the N-terminus of oligo and polypeptides. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, T cell activation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. MW: ~210000-260000 (unreduced).
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmole 7-(Gly-Pro)-amino-4-methylcoumarinamide per min at 37°C, pH 8.5.
Physical form
In 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.
Reconstitution
Dilute only the amount of enzyme needed for each assay. Upon thawing, do not re-freeze, store in the refrigerator (4°C).
Other Notes
Bar, J., et al. 2003. Biol. Chem.384, 1553.
Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA100, 5063.
Ikehara, Y., et al. 1994. Methods. Enzymol.244, 215.
David, F., et al. 1993. J. Biol. Chem.268, 17247.
Thomsen, P.D., et al. 1993. Mamm. Genome4, 604.
Misumi, Y., et al. 1992. Biochim. Biophys. Acta.1131, 333.
Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler372, 213.
Checler, F., et al. 1985. J. Neurochem.45, 1509.
Imai, K., et al. 1983. J. Biochem.93, 431.
Kato, T., et al. 1979. Experientia.35, 409.
Kojima, K., et al. 1979. Anal. Biochem.100, 43.
Kato, T., et al. 1978. Biochem. Med.19, 351.
Kenny, A.J., et al. 1976. Biochem. J.157, 169.
Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA100, 5063.
Ikehara, Y., et al. 1994. Methods. Enzymol.244, 215.
David, F., et al. 1993. J. Biol. Chem.268, 17247.
Thomsen, P.D., et al. 1993. Mamm. Genome4, 604.
Misumi, Y., et al. 1992. Biochim. Biophys. Acta.1131, 333.
Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler372, 213.
Checler, F., et al. 1985. J. Neurochem.45, 1509.
Imai, K., et al. 1983. J. Biochem.93, 431.
Kato, T., et al. 1979. Experientia.35, 409.
Kojima, K., et al. 1979. Anal. Biochem.100, 43.
Kato, T., et al. 1978. Biochem. Med.19, 351.
Kenny, A.J., et al. 1976. Biochem. J.157, 169.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service