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287815

Sigma-Aldrich

3,4-Dichloroisocoumarin

Synonym(s):

3,4-Dichloroisocoumarin

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About This Item

Empirical Formula (Hill Notation):
C9H4Cl2O2
CAS Number:
51050-59-0
Molecular Weight:
215.03
MDL number:
MFCD00036960
UNSPSC Code:
12352200
NACRES:
NA.77

Assay

≥99% (elemental analysis)

Quality Level

100

form

lyophilized solid

potency

5-100 μM IC50

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

white

solubility

DMF: 10 mM
DMSO: 10 mM

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C9H4Cl2O2/c10-7-5-3-1-2-4-6(5)9(12)13-8(7)11/h1-4H

InChI key

SUGXUUGGLDCZKB-UHFFFAOYSA-N

General description

A potent irreversible inhibitor of serine proteases. Blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. Reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. Does not affect thiol proteases and metalloproteases. Also has no activity towards β-lactamases. Effective at concentrations ranging from 5-100 µM.
A potent, irreversible inhibitor of serine proteases. Reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. Inhibits granzyme B and blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. Does not affect thiol proteases and metalloproteases. Does not exhibit any activity towards β-lactamases.
Effective concentration: 5-100 µM; t½ = 20 min at pH 7.5.

Biochem/physiol Actions

Cell permeable: no
Primary Target
serine proteases
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Cain, K., et al. 1994. Biochem. Cell Biol.72, 631.
Weaver, V.M., et al. 1993. Biochem. Cell Biol. 71, 488.
Odake, S., et al. 1991. Biochemistry 30, 2217.
Harper, J.W., et al. 1985. Biochemistry24, 1831.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H301,H315,H319,H335

Hazard Classifications

Acute Tox. 3 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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