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Merck
CN

264150

Caspase-3 Substrate IV, Fluorogenic

Fluorogenic caspase-3 substrate.

Synonym(s):

Caspase-3 Substrate IV, Fluorogenic, Z-DEVD-AFC

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About This Item

Empirical Formula (Hill Notation):
C36H38F3N5O14
CAS Number:
1177935-21-5
Molecular Weight:
821.71
MDL number:
MFCD01074977
UNSPSC Code:
12352202
NACRES:
NA.77

Key Documents

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Product Name

Caspase-3 Substrate IV, Fluorogenic, Fluorogenic caspase-3 substrate.

InChI key

NLDGPABVDARZCY-KIAQLKRMSA-N

SMILES string

FC(F)(F)C1=CC(=O)Oc2c1ccc(c2)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)OCc3ccccc3)CC(=O)OC)CCC(=O)OC)C(C)C)CC(=O)OC

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white to off-white

solubility

DMSO: 10 mg/mL

fluorescence

λex ~400 nm
λem ~505 nm

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Cell permeable: no
Primary Target
Fluorogenic caspase-3 substrate.
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Standard Handling (A)

General description

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.
Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Other Notes

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.
Z-Asp-Glu-Val-Asp-AFC

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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