Skip to Content
Merck
CN
All Photos(1)

Documents

234196

Sigma-Aldrich

Anti-Collagen Type X Rabbit pAb

liquid, Calbiochem®

Sign Into View Organizational & Contract Pricing

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

unmodified

Gene Information

human ... COL10A1(1300)

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes type X collagen.
Recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen.
This Anti-Collagen Type X Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Collagen Type X.

Immunogen

Rat
purified, rat chondrosarcoma cell collagen type X

Application

Frozen Sections (see comments)

Immunoblotting (1:100-1:300)

Immunoprecipitation (1:20-1:40)

Paraffin Sections (see application reference; pretreatment required)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Chung, K.S., et al. 1995. Dev. Biol. 170, 387.
Schmid. T.M., and Linsenmayer, T.M. 1985. Dev. Biol. 107, 373.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Karthikeyan Rajagopal et al.
Cartilage, 13(2_suppl), 571S-581S (2021-09-29)
Hypertrophic cartilage formation is a major setback in mesenchymal stem cells (MSCs)-mediated cartilage repair, and overcoming it requires optimization of differentiation. Here, we tested the miR-140 activated collagen hydrogel for the chondrogenic differentiation of MSCs and to produce hyaline cartilage.
Carmen Corciulo et al.
Nature communications, 8, 15019-15019 (2017-05-12)
Osteoarthritis (OA) is characterized by cartilage destruction and chondrocytes have a central role in this process. With age and inflammation chondrocytes have reduced capacity to synthesize and maintain ATP, a molecule important for cartilage homeostasis. Here we show that concentrations
Michael M Weinstein et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 29(8), 1815-1822 (2014-03-20)
Activating mutations in transient receptor potential vanilloid family member 4 (Trpv4) are known to cause a spectrum of skeletal dysplasias ranging from autosomal dominant brachyolmia to lethal metatropic dysplasia. To develop an animal model of these disorders, we created transgenic
Karthikeyan Rajagopal et al.
Bone & joint research, 9(9), 601-612 (2020-10-06)
Extracellular matrix (ECM) and its architecture have a vital role in articular cartilage (AC) structure and function. We hypothesized that a multi-layered chitosan-gelatin (CG) scaffold that resembles ECM, as well as native collagen architecture of AC, will achieve superior chondrogenesis
Marjolein M J Caron et al.
PloS one, 11(4), e0153162-e0153162 (2016-04-07)
NSAIDs are used to relieve pain and decrease inflammation by inhibition of cyclooxygenase (COX)-catalyzed prostaglandin (PG) synthesis. PGs are fatty acid mediators involved in cartilage homeostasis, however the action of their synthesizing COX-enzymes in cartilage differentiation is not well understood.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service