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227041

Sigma-Aldrich

Cholera Toxin, B Subunit, Type Inaba 569B, Peroxidase Conjugate

peroxidase conjugate, Suitable for the demonstration of dendritic branching in retrogradely labeled neurons.

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UNSPSC Code:
12352202

conjugate

peroxidase conjugate

Quality Level

form

solid

specific activity

≥125 purpurogallin units/mg protein (HRP)

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

shipped in

ambient

storage temp.

2-8°C

General description

Sold on the basis of HRP content.
Suitable for the demonstration of dendritic branching in retrogradely labeled neurons. Has been shown to be up to 50 times more sensitive than free peroxidase as both an orthogradely and retrogradely transported marker in the rat visual system.
Suitable for the demonstration of dendritic branching in retrogradely labeled neurons. Shown to be up to 50 times more sensitive than free peroxidase as both an orthogradely and retrogradely transported marker in the rat visual system.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Demonstration of dendritic branching in retrogradely labeled neurons
Product does not compete with ATP.
Reversible: no

Packaging

yes

Warning

Toxicity: Harmful (C)

Physical form

Lyophilized from 10 mM sodium phosphate buffer, pH 7.5.

Preparation Note

Prepared by a modification of the two-step conjugation method of Avrameas, S., and Ternynck, T. 1971. Chromatographically purified and lyophilized.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Reconstitute in ≤100 µl of sterile distilled H₂O.

Other Notes

Raappana, P., and Arvidsson, J. 1993. J. Comp. Neurol. 328, 103.
Trojanowski, J.Q. 1983. J. Neurosci. Methods9, 185.
Trojanowski, J.Q., et al. 1982. Brain Res.231, 33.
Wan, X.S.T., et al. 1982. Exp. Neurol.78, 167.
Avrameas, S., and Ternynck, T. 1971. Immunochemistry8, 1175.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Resp. Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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