227036
Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free
Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins.
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Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free
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Quality Level
Assay
≥95% (SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
shipped in
ambient
storage temp.
2-8°C
General description
Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface.
Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface..
Useful for tissue culture applications.
Biochem/physiol Actions
Cell permeable: no
Primary Target
α-subunit of G-proteins
α-subunit of G-proteins
Product does not compete with ATP.
Reversible: no
Packaging
Please refer to vial label for lot-specific concentration.
yes
Warning
Toxicity: Highly Toxic (H)
Physical form
Lyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.
Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.
Analysis Note
Binding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.
single band by disc gel electrophoresis
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Gilman, A.G. 1987. Annu. Rev.Biochem.56, 615.
Legal Information
Not available for sale outside of the United States.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Skin Irrit. 2
WGK
WGK 3
Regulatory Information
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Certificates of Analysis (COA)
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