219350
Anti-β-Catenin, Dephospho Mouse mAb (8E4)
lyophilized, clone 8E4, Calbiochem®
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
8E4, monoclonal
form
lyophilized
species reactivity
human, rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
General description
Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~90 kDa nuclear dephospho β-catenin protein. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
Recognizes the ~90 kDa nuclear dephospho-β-catenin in vanadate treated A431 cells. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
This Anti-β-Catenin, Dephospho Mouse mAb (8E4) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of β-Catenin, Dephospho.
Immunogen
Mouse
a synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH
Application
ELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml colorimetric, 0.1 µg/ml, chemiluminescence)
Immunoprecipitation (1-10 µg/10⁶ LiCl-treated SW480 cells)
Immunoblotting (1 µg/ml colorimetric, 0.1 µg/ml, chemiluminescence)
Immunoprecipitation (1-10 µg/10⁶ LiCl-treated SW480 cells)
Warning
Toxicity: Standard Handling (A)
Physical form
100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.
Reconstitution
Reconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
LiCl-treated SW480 cells (included)
LiCl-treated SW480 cells (included)
Other Notes
SW480 LiCl cell lysate is recommended for immunoblot applications at a concentration of 20 µl/lane (mini gel) for chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service