218701
Casein Kinase II, Human, Recombinant, E. coli
Casein Kinase II, Human, Recombinant, E. coli Recombinant, is expressed in E. coli. A cyclic nucleotide-independent Ser/Thr protein kinase composed of catalytic α-subunits and regulatory β-subunits.
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Synonym(s):
rhCKII
UNSPSC Code:
12352202
NACRES:
NA.77
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Research area: CELL SIGNALING
Note: 1 KU = 1000 units.
Recombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits and regulatory β-subunits in an α2β2- tetramer. This material is one of several forms of native CK-II. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies. The following substrates have been characterized by CK-II both in vitro and in vivo: nucleolin, RNA polymerase, p53, and SV 40 large T antigen.
Note: 1 KU = 1000 units.
Recombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits and regulatory β-subunits in an α2β2- tetramer. This material is one of several forms of native CK-II. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies. The following substrates have been characterized by CK-II both in vitro and in vivo: nucleolin, RNA polymerase, p53, and SV 40 large T antigen.
Application
Casein kinase II (CK2) has been used:
- has been used as a marker for CK2 subunits, to analyze CK2 expression in human retinal extract.
- to investigate the phosphorylation of the C-terminal domain of uroplakin IIIa and assess its potential regulation by CK2.
- to investigate the phosphorylation events and their impact on spectrin tetramer formation and neuronal cell function.
Biochem/physiol Actions
Casein kinase II (CK2) is an active serine/threonine protein kinase that plays a pivotal role in cell growth and division. It is responsible for phosphorylating various nuclear proteins involved in DNA metabolism, transcription factors, and oncogenes, thus influencing their activity. It also regulates osteogenesis, adipogenesis, chondrogenesis, neuron differentiation, and immune response pathways. CK2 plays a crucial role in activating the Janus Kinase/signal transducer and activator of transcription (JAK/STAT) pathway, which contributes to cell survival, differentiation, and proliferation. Furthermore, CK2 is involved in activating the nuclear factor kappa B (NF-κB) pathway. It is highly expressed in different hematological malignancies, including acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), and chronic myeloid leukemia (CML).
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl.
Physical form
In 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON™ X-100 Detergent, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Yamaguchi, Y., et al. 1998. Nucleic Acids Res.26, 3854.
Chester, N., and Marshak, D.R. 1993. Anal. Biochem.209, 284.
Russo, G.L., et al. 1992. J. Biol. Chem.267, 20317.
Chester, N., and Marshak, D.R. 1993. Anal. Biochem.209, 284.
Russo, G.L., et al. 1992. J. Biol. Chem.267, 20317.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Casein kinase II in signal transduction and cell cycle regulation
Litchfield DW and Luscher B
Molecular and Cellular Biochemistry (1993)
Matthew Reid MacPherson et al.
Molecular biology of the cell, 21(2), 244-253 (2009-11-20)
Snail1 is a major factor for epithelial-mesenchymal transition (EMT), an important event in tumor metastasis and in other pathologies. Snail1 is tightly regulated at transcriptional and posttranscriptional levels. Control of Snail1 protein stability and nuclear export by GSK3beta phosphorylation is
Proteolysis-targeting chimeras (PROTACs) as novel biotechnology for cancer therapy
Ota Fuchs
Journal of Separation Science, 71-88 (2022)
Converting GTP hydrolysis into motion: versatile translational elongation factor G
Rodnina MV, et al.
Biological Chemistry, 401(1), 131-142 (2019)
Bacteria-induced uroplakin signaling mediates bladder response to infection
Thumbikat P, et al.
PLoS Pathogens, 5(5), e1000415-e1000415 (2009)
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