208773
Calpain Substrate IV, Cell-Permeable, Fluorogenic
The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.
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Synonym(s):
Calpain Substrate IV, Cell-Permeable, Fluorogenic
Empirical Formula (Hill Notation):
C127H206N50O27S
Molecular Weight:
2897.38
UNSPSC Code:
12352200
NACRES:
NA.77
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Quality Level
Assay
≥95% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
protect from light
solubility
DMSO: 5 mg/mL
fluorescence
λex 480 nm; λem 320 nm
shipped in
ambient
storage temp.
−20°C
General description
The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Sequence
(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂
Physical form
Supplied as a trifluoroacetate salt
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Banoczi, Z., et al. 2008. Bioconjug. Chem.19, 1375.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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