208694
Calmodulin, Bovine Brain, High Purity
Calmodulin is a ubiquitous Ca2+-binding protein that serves as a physiological effector of a wide range of biological processes. Purified from bovine brain.
Synonym(s):
Calmodulin, Bovine Brain, High Purity, CaM, Ca 2+/Calmodulin, CaM, Ca2+/Calmodulin
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About This Item
biological source
bovine brain
Quality Level
Assay
≥95% (SDS-PAGE)
form
lyophilized
specific activity
≥250,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
cell based assay: suitable
solubility
aqueous buffer: soluble
water: soluble
shipped in
wet ice
storage temp.
−20°C
Gene Information
bovine ... Calm(32659)
General description
Research area: Cell Signaling
Calmodulin (CaM) ranks among the most highly conserved protein sequences, with only histone proteins H4 and H3, actin B, and ubiquitin showing greater evolutionary conservation. CaM is a primarily helical protein with N- and C-terminal domains, each containing two paired Ca2+-binding EF-hand motifs. These motifs, with pentagonal-bipyramidal geometry, feature a conserved 12-amino acid sequence (positions 1–12) within a canonical helix-loop-helix structure.
Calmodulin (CaM) ranks among the most highly conserved protein sequences, with only histone proteins H4 and H3, actin B, and ubiquitin showing greater evolutionary conservation. CaM is a primarily helical protein with N- and C-terminal domains, each containing two paired Ca2+-binding EF-hand motifs. These motifs, with pentagonal-bipyramidal geometry, feature a conserved 12-amino acid sequence (positions 1–12) within a canonical helix-loop-helix structure.
Application
Calmodulin, Bovine Brain, High Purity has been used to study melatonin-calmodulin interaction and to activate Calcium–calmodulin (CaM)-dependent protein kinase IIa (CaMKII)a through in vitro incubation.
Biochem/physiol Actions
Calmodulin (CaM) is a ubiquitous Ca2+-binding protein distributed in eukaryotic cells that serves as a major physiological effector for a wide range of cellular responses. The Ca2+-calmodulin complex is involved in the maintenance of intracellular Ca2+ homeostasis, as well as in the regulation of the intracellular concentration of other second messengers, such as cyclic AMP and inositol trisphosphate. Mutations in the CaM sequence can result in diverse and potentially life-threatening pathologies such as ventricular tachycardia, congenital arrhythmia, and long QT syndrome. These mutations have the capacity to modify CaM′s ability to bind to Ca2+ and its target proteins and peptides, thereby disrupting their subsequent activity. Ca2+/CaM complex and CaM-binding domains (CaMBDs) interactions influence memory formation, muscle contraction, cellular metabolism, and cytoskeletal rearrangements.
Packaging
Yes
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of calmodulin that gives rise to 50% of the maximal enzyme activation of a standard level of activator-deficient calcineurin.
Physical form
Lyophilized from 30 mM HEPES, 1 mM CaCl₂, 0.1 mM DTT, pH 7.4.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C or -70°C). Aqueous stock solutions are stable for up to 2 months at -20°C or for up to 4 months at -70°C.
Please refer to the vial label for lot-specific reconstitution volume.
Other Notes
This product is not to be used for animal treatment, in vivo research or in any other contact procedure with livestock.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
Certificates of Analysis (COA)
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Olivia Appelmans et al.
Analytical biochemistry, 630, 114313-114313 (2021-07-25)
Radiometric assays have widely been used for measuring protein kinase activity for decades. In addition, several non-radiometric kinase assay formats have been developed over the years, including luciferase-based and fluorescence-based assays. However, radiometric assays are still considered as the "gold
Corey Andrews et al.
International journal of molecular sciences, 22(1) (2021-01-06)
Calmodulin (CaM) is an important intracellular protein that binds Ca2+ and functions as a critical second messenger involved in numerous biological activities through extensive interactions with proteins and peptides. CaM's ability to adapt to binding targets with different structures is
Jesús Argueta et al.
International journal of molecular sciences, 23(5) (2022-03-11)
Melatonin (MEL) is a pleiotropic indolamine that reaches multiple intracellular targets. Among these, MEL binds to calmodulin (CaM) with high affinity. In presence of Ca2+, CaM binds to CaM-dependent kinase II (CaMKII). The Ca2+-CaM/CaMKII pathway regulates a myriad of brain
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