207000
Calcineurin Autoinhibitory Peptide
The Calcineurin Autoinhibitory Peptide controls the biological activity of Mn2+-stimulated calcineurin activity. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.
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Synonym(s):
Calcineurin Autoinhibitory Peptide, ITSFEEAKGLDRINERMPPRRDAMP
Empirical Formula (Hill Notation):
C124H205N39O39S2
Molecular Weight:
2930.32
UNSPSC Code:
12352202
Recommended Products
Quality Level
Assay
≥97% (HPLC)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
solubility
water: soluble
shipped in
ambient
storage temp.
−20°C
General description
Corresponds to a C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin. Specific calcineurin inhibitor. Does not inhibit CaM kinase II, protein phosphatase I or 2A. Inhibits Mn2+-stimulated calcineurin activity (IC50 = 10 µM using 32P myosin light chain as a substrate) but has no effect on Ni2+-stimulated activity.
Specific calcineurin inhibitor. Corresponds to the C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin (PP2B). Inhibits Mn2+-stimulated calcineurin activity (IC50 = 10 µM using 32P-myosin light chain as a substrate) but has no effect on Ni2+-stimulated enzyme activity. Does not inhibit CaM kinase II, protein phosphatase 1 or 2A.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Mn2+-stimulated calcineurin activity
Mn2+-stimulated calcineurin activity
Product does not compete with ATP.
Reversible: no
Target IC50: 10 µM using 32P-myosin light chain as a substrate
Packaging
Yes
Warning
Toxicity: Standard Handling (A)
Sequence
H-Ile-Thr-Ser-Phe-Glu-Glu-Ala-Lys-Gly-Leu-Asp-Arg-Ile-Asn-Glu-Arg-Met-Pro-Pro-Arg-Arg-Asp-Ala-Met-Pro-OH
Physical form
Supplied as a trifluoroacetate salt.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions are stable for up to 2 months at -20°C.
Other Notes
Perrino, B.A., et al. 1995. J. Biol. Chem. 270, 340.
Yokoyama, Y., and Wang, J.H. 1994. FEBS Lett.337, 128.
Yokoyama, N., et al. 1993. Arch. Biochem. Biophys.300, 615.
Matsui, H., et al. 1991. Biochem. Int.24, 1119.
Hashimoto, Y., et al. 1990. J. Biol. Chem.265, 1924.
Yokoyama, Y., and Wang, J.H. 1994. FEBS Lett.337, 128.
Yokoyama, N., et al. 1993. Arch. Biochem. Biophys.300, 615.
Matsui, H., et al. 1991. Biochem. Int.24, 1119.
Hashimoto, Y., et al. 1990. J. Biol. Chem.265, 1924.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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