204860
Complement C3b, Human
Complement C3b, Human, is a native C3b complement component. It is a glycoprotein composed of the modified C3-α chain (αʹ) (M.W. 105,000) and the intact C3-β chain (M.W. 75,000).
Synonym(s):
C3b, Complement C3
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.25
Quality Level
Assay
≥90% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
impurities
IgG, IgA, IgM, C5, Factor H, or Factor I, trace
shipped in
wet ice
storage temp.
−70°C
General description
Native, human C3b complement component. Cleavage of the C3-α chain at peptide bond 77 by either of the complement C3 convertase enzymes results in production of C3a (M.W. 9083) and C3b (M.W. 180,000) fragments. The C3b fragment is a glycoprotein composed of the modified C3-α chain (α′) (M.W. 105,000) and the intact C3-β chain (M.W. 75,000). Nascent C3b has the transient ability to form a covalent ester bond with a variety of target surfaces. Once bound to target surfaces C3b becomes an essential subunit of both the classical and alternative pathway C5 cleaving enzymes. In addition, surface-bound C3b has opsonic and immune adherence activities which are mediated via binding to CR1 (CD35) complement receptors.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, pH 7.2, filtered through a 0.22 µm filter.
Preparation Note
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Wong, W.W. and Fearon, D.T. 1987. Methods Enzymol.150, 579.
Arnout, M.A., et al. 1981. J. Immunol.127, 1348.
Arnout, M.A., et al. 1981. J. Immunol.127, 1348.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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