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19-160

Sigma-Aldrich

BrdU (5-Bromo-2′-deoxyuridine)

Used for the study of DNA synthesis where it is incorporated into DNA in place of thymidine, BrdU can be used in conjunction with anti-BrdU for immunocytochemical analysis of cell proliferation.

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UNSPSC Code:
41116012
eCl@ss:
32160405
NACRES:
NA.41

Quality Level

100

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable

shipped in

wet ice

Application

Research Category
All
Used for the study of DNA synthesis where it is incorporated into DNA in place of thymidine; BrdU can be used in conjunction with anti-BrdU for immunocytochemical analysis of cell proliferation.

Physical form

C9H11BrN2O5

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H340,H361fd

Hazard Classifications

Muta. 1B - Repr. 2

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is contained in Product 19160, SOD Assay Kit (Superoxide dismutase)?

    This kit contains WST Solution (1x5mL), Enzyme Solution (1x100μL), Buffer Solution (1x100mL), Dilution Buffer (1x50mL), and a manual.  

  4. What equipment is required to use Product 19160, SOD Assay Kit (Superoxide dismutase)?

    The equipment needed to use this kit is a plate reader (450 nm filter), a 96-well microplate, 10 μL & 100-200 μL pipettes, a multichannel pipette and an incubator. Superoxide dismutase (SOD) is also necessary for the preparation of an inhibition curve.

  5. How do I prepare a WST working solution for Product 19160, SOD Assay Kit (Superoxide dismutase)?

    Dilute 1mL of WST Solution with 19mL of Buffer Solution.

  6. When using Product 19160, SOD Assay Kit (Superoxide dismutase), how do I prepare an Enzyme working solution?

    Centrifuge the Enzyme Solution tube for five seconds.  Mix by pipeting, and dilute 15μL of Enzyme Solution with 2.5mL of Dilution Buffer.

  7. When using Product 19160, SOD Assay Kit, how do I prepare a Superoxide Dismutase (SOD) working solution?

    Dilute SOD with Dilution Buffer to prepare SOD standard Solutions if necessary for assay monitoring as follows:  200 U/mL, 100 U/mL, 50 U/mL, 20 U/mL, 10 U/mL, 5 U/mL, 1 U/mL, 0.1 U/mL, 0.05 U/mL, 0.01 U/mL, and 0.001 U/mL.

  8. Are there any literature references for Product 19160, SOD Assay Kit (Superoxide dismutase)?

    1. J. M. McCord and I. Fridovich, J. Biol. Chem., 244, 6049 (1969).  2. S. Goldstein and G. Czapski, Free Rad. Res. Comms.,12-13(Part 1), 5-10 (1991).  3. R. H. Burdon, V. Gill, and C. Rice-Evans, Free Rad. Res. Comms., 18, 369 (1993).  4. M. W. Sutherland and B. Learmonth, Free Rad. Res., 27, 283 (1997).  5. H. Ukeda, A. K. Sarker, D. Kawana and M. Sawamura, Anal. Sci., 15, 353 (1999).  6. H. Ukeda, D. Kawana, S. Maeda and M. Sawamura, Biosci. Biotechnol. Biochem., 63, 485 (1999).  7. A. V. Peskin, et al., Clin. Chim. Acta., 293, 157 (2000).

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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