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form
lyophilized solid
Quality Level
specific activity
≥10 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
shipped in
ambient
storage temp.
−20°C
General description
A high affinity biotin binding protein (Kd approximately 10-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.
A high-affinity biotin binding protein.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
Reconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.
Other Notes
Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
Roth, J., et al. 1992. Histochemistry 98, 229.
Sano, T., et al. 1992. Science 258, 120.
Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
Srivastava, P., et al. 1992. Cytometry 13, 711.
Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
Roth, J., et al. 1992. Histochemistry 98, 229.
Sano, T., et al. 1992. Science 258, 120.
Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
Srivastava, P., et al. 1992. Cytometry 13, 711.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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