Skip to Content
Merck
CN
All Photos(1)

Documents

Safety Information

17-294

Sigma-Aldrich

Rho Activation Assay Kit

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable
affinity binding assay: suitable

NCBI accession no.

UniProt accession no.

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

dry ice

Gene Information

human ... RHOA(387)

General description

A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.

Application

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

Packaging

Kit capacity: 30 assays

Components

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)

Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)

Quality

Routinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

WGK

WGK 3

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

M P Gratacap et al.
The Journal of biological chemistry, 276(51), 47906-47913 (2001-09-19)
Platelets were used to study the activation of Rho and Rac through G-protein-coupled receptors and its regulation by cyclic nucleotides. The thromboxane A(2) (TXA(2)) mimetic rapidly activated both small GTPases independently of integrin alpha(IIb)beta(3) activation., which leads to the activation
Toshifumi Sugatani et al.
The Journal of biological chemistry, 278(7), 5001-5008 (2002-12-04)
PTEN (also known as MMAC-1 or TEP-1) is a frequently mutated tumor suppressor gene in human cancer. PTEN functions have been identified in the regulation of cell survival, growth, adhesion, migration, and invasiveness. Here, we characterize the diverse signaling networks
Geraldine Pawlak et al.
The Journal of biological chemistry, 277(30), 26927-26933 (2002-05-16)
Cellular transformation by v-Src is believed to be caused by aberrant activation of signaling pathways that are normally regulated by cellular Src. Using normal rat kidney cells expressing a temperature-sensitive mutant of v-Src, we examined the role of the Raf/MEK/ERK
Y Yamaguchi et al.
The Journal of biological chemistry, 276(22), 18977-18983 (2001-03-30)
The Rho family of small GTPases has been shown to be involved in the regulation of neuronal morphology, and Rac and Rho exert antagonistic actions in neurite formation. In this study, we have examined the cross-talk between Rac and Rho
Mark S Wainwright et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(10), 6233-6238 (2003-05-06)
Acute lung injury (ALI) associated with sepsis and iatrogenic ventilator-induced lung injury resulting from mechanical ventilation are major medical problems with an unmet need for small molecule therapeutics. Prevailing hypotheses identify endothelial cell (EC) layer dysfunction as a cardinal event

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service