171372
Amphiregulin, Human, Recombinant
Amphiregulin, Human, Recombinant, consisting of amino acids 101-198 and is expressed in E. coli. Stimulates the proliferation of various human and mouse keratinocytes.
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Synonym(s):
Schwannoma-derived growth factor (SDGF)
UNSPSC Code:
12352203
NACRES:
NA.77
Recommended Products
recombinant
expressed in E. coli
Quality Level
Assay
≥97% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, human amphiregulin (AR) consisting of amino acids 101-198 and expressed in E. coli. Human amphiregulin is able to stimulate the proliferation of various human and mouse keratinocytes, mammary epithelial cells, and some fibroblasts. Shown to be an autocrine growth factor, amphiregulin is also a growth inhibitor for various tumor cells and can bind to the EGF receptor.
Biochem/physiol Actions
ED₅₀ = 5-15 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 0.2 µm sterile-filtered PBS, BSA.
Reconstitution
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
Certificates of Analysis (COA)
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