17-10077
Magna ChIP® HT96 Chromatin Immunoprecipitation Kit
The Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.
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About This Item
Quality Level
manufacturer/tradename
Magna ChIP®
technique(s)
immunoprecipitation (IP): suitable
shipped in
dry ice
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General description
Features & Benefits
- Complete set of materials for up to 96 ChIP reactions
- Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone
- Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction
- Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systems
- Protocols for using cells or tissues
- Direct analysis of DNA without additional clean-up steps
- Compatible with ChIPAb+ validated antibody and primer sets
Application
Epigenetics & Nuclear Function
Packaging
Physical form
Preparation Note
Other Notes
HT96 Nuclei Isolation Buffer
HT96 ChIP Buffer (Sonication/ChIP/Wash)
Low Stringency IP Wash Buffer
HT96 ChIP Elution Buffer
Proteinase K Solution
Protease Inhibitor Cocktail III
10X Glycine
10X PBS
96 Well ChIP Plate
96 Well Thermal Plate
Plate Seal
Strip Caps
Legal Information
Disclaimer
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10-13 - German Storage Class 10 to 13
WGK
WGK 3
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Related Content
Magna ChIP™ HT96 Kits for high throughput chromatin immunoprecipitation
Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA- and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. In a typical ChIP experiment either protein A or G is selected for enrichment depending on the antibody isotype. However, proteins A and G possess differing affinities for human and mouse IgGs. Complicating this choice, for some antibody isotypes there is affinity for both protein A and G. In addition, we have observed that independent of the isotype the affinity of a specific antibody for protein A or G can vary depending on the specific clone, purification method, and source.
Superior enrichment, low background. With performance proven for both qPCR and ChIP-seq analysis, the Magna ChIP™ HiSens kit may be the only ChIP kit you’ll ever need. Outperforming any competing kit, this revolutionary approach to ChIP enables enrichment from both low and high amounts of input chromatin while also delivering low backgrounds and high signal-to-noise ratios for ultra-sensitive detection.
The 3rd edition of An Introduction to Antibodies and Their Applications provides a concise overview of some of the key features for the use of antibodies and immunochemical techniques in biological research. This handy reference guide supplements the techniques described in literature, recorded in general laboratory procedures, and described on individual product data sheets. Antibody design, development, and production are our expertise. Stringent validation of our antibodies is only one component of a comprehensive process we undertake to provide the antibodies most cited by the research community (see section Antibody Quality on page 2 for an in-depth look at our expertise).
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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