Skip to Content
Merck
CN

16-663

Magna ChIP® Protein A+G Magnetic Beads

provides a rapid, reproducible and efficient collection of immunocomplexes for ChIP and RIP assays

Synonym(s):

ChIP magnetic A+G beads, ChIP magnetic A/G beads, ChIP magnetic beads

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.84
UNSPSC Code:
41105501

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

packaging

pkg of 1 mL

manufacturer/tradename

Magna ChIP®

storage condition

do not freeze

particle size

~3 μm

shipped in

wet ice

storage temp.

2-8°C

Quality Level

100

General description

Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. Experiments comparing protein A vs. protein G vs. protein A/G magnetic bead blends revealed that a mixture of protein A and G beads worked well with a wide variety of antibody isotypes. The use of Protein A/G bead blends eliminated the need to consider which beads or kit to use in order match a particular antibody/bead binding affinity combination. In addition to simplifying the procedure, comparing the use of either protein A or protein G alone, protein A/G magnetic bead blends improved signal-to-noise ratios without decreasing the recovery of input chromatin in ChIP assays.

Application

This blend of protein A+G magnetic beads allows for the use of a wider range of antibodies than A or G alone & provides a rapid, reproducible & efficient collection of immunocomplexes for chromatin immunoprecipitations (ChIP) and RNA immunoprecipitations (RIP) assays.

Use 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex.

Used to detect/quantify: Protein A+G

Physical form

Liquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of shipment. Do Not Freeze.

Analysis Note

Routinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP® A Kit (Cat. #17-610).

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Getting started with ChIP-seq: experimental design to data analysis

Chromatin-immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-seq) of the immunoprecipitated DNA is a powerful tool for the investigation of protein:DNA interactions. To perform ChIP-seq, chromatin is isolated from cells or tissues (with or without chemical crosslinking) and fragmented. Antibodies recognizing chromatinassociated proteins of interest are used to enrich the sample for specific chromatin fragments. The DNA is recovered, sequenced on various NGS platforms, and aligned to a reference genome to determine specific protein binding loci. ChIP-seq studies have increased our knowledge of transcription factor biology, DNA methylation and histone modifications.

New Products: Volume 3, 2012

New Products: Antibodies, Assays, Small Molecules, Inhibitors, and Proteins

Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals

Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA- and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. In a typical ChIP experiment either protein A or G is selected for enrichment depending on the antibody isotype. However, proteins A and G possess differing affinities for human and mouse IgGs. Complicating this choice, for some antibody isotypes there is affinity for both protein A and G. In addition, we have observed that independent of the isotype the affinity of a specific antibody for protein A or G can vary depending on the specific clone, purification method, and source.

PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD
See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service