16-455
Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate
clone 4A4, Upstate®, from mouse
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Synonym(s):
4A4, anti-phosphoserine, phosphoserine antibody, pan-phosphoserine
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
4A4, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
phosphorylation (pSer)
General description
The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotrans-mission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that over 95% of all phosphorylation events occur on serine and threonine residues.
Specificity
Recognizes proteins containing phosphoserine residues, regardless of origin.
Immunogen
Epitope: phosphoserine
Phosphoserine conjugated to KLH.
Application
Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate is an antibody against Phosphoserine for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Quality
Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.
Target description
Varies
Physical form
100 μL of protein G purified mouse IgG1 in PBS containing 0.05% Kathon® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:4000 in 10 mL blocking buffer. Liquid.
Protein G Purified
Storage and Stability
6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A Po et al.
Oncogene, 36(32), 4641-4652 (2017-04-04)
Aberrant Hedgehog/GLI signaling has been implicated in a diverse spectrum of human cancers, but its role in lung adenocarcinoma (LAC) is still under debate. We show that the downstream effector of the Hedgehog pathway, GLI1, is expressed in 76% of
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