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16-256

Anti-Phosphatidylserine Antibody, clone 1H6, Alexa Fluor^™ 488

Name: Anti-Phosphatidylserine Antibody, clone 1H6, Alexa Fluor<SUP>™</SUP> 488
Brand: MM

clone 1H6, Upstate^®, from mouse

Synonym(s):

488-Alexa Fluor Antibody, Alexa Fluor 488 Detection, Clone 1H6 Anti-Phosphatidylserine

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR^™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1H6, monoclonal

species reactivity

vertebrates

manufacturer/tradename

Upstate^®

technique(s)

flow cytometry: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

phosphorylation (pSer)

Related Categories

Flow Cytometry Antibodies

General description

Significance:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.

Phosphatidylserine, or PS, is a naturally occurring, phospholipid nutrient. PS is essential to the functioning of all the cells of the body, but is most concentrated in the brain. Its relative abundance in this organ reflects its proven involvement in an assortment of nerve cell functions, including nerve transmitter release and synaptic activity. Clinical studies have suggested that PS can support brain functions that tend to decline with age.

Specificity

Recognizes phosphatidylserine (PS) in cell membranes.

Immunogen

Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol.

Application

Detect Phosphatidylserine using this Anti-Phosphatidylserine Antibody, clone 1H6, Alexa Fluor 488 validated for use in FC.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Flow Cytometry: 0.2 μg of this antibody detected Phosphatidylserine in fixed Jurkat cells (see page 2).

Apoptosis Assay: Time course for induction of apoptosis in Jurkat cells by staurosporine, measured using either an Anti-Phosphatidylserine, clone 1H6, Alexa Fluor^™ 488 conjugate or an Annexin V FITC conjugate.

Physical form

Protein G Purified

Purified mouse monoclonal IgG in buffer containing PBS containing 1% BSA, 0.05% Tween, 0.05% sodium azide. Liquid at 4ºC.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor^™ 488-conjugate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor^™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas.

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Scientific reports, 11(1), 6392-6392 (2021-03-20)

Head and neck squamous cell carcinomas (HNSCC) induced by human papillomavirus (HPV) have increased recently in the US. However, the distinct alterations of molecules involved in the death pathways and drug effects targeting inhibitor of apoptosis proteins (IAPs) have not

Vagus nerve stimulation primes platelets and reduces bleeding in hemophilia A male mice.

Carlos E Bravo-Iñiguez et al.

Nature communications, 14(1), 3122-3122 (2023-06-02)

Deficiency of coagulation factor VIII in hemophilia A disrupts clotting and prolongs bleeding. While the current mainstay of therapy is infusion of factor VIII concentrates, inhibitor antibodies often render these ineffective. Because preclinical evidence shows electrical vagus nerve stimulation accelerates

Tumor-derived extracellular vesicles regulate tumor-infiltrating regulatory T cells via the inhibitory immunoreceptor CD300a.

Yuta Nakazawa et al.

eLife, 10 (2021-11-10)

Although tumor-infiltrating regulatory T (Treg) cells play a pivotal role in tumor immunity, how Treg cell activation are regulated in tumor microenvironments remains unclear. Here, we found that mice deficient in the inhibitory immunoreceptor CD300a on their dendritic cells (DCs)

A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V.

I Vermes et al.

Journal of immunological methods, 184(1), 39-51 (1995-07-17)

In the early stages of apoptosis changes occur at the cell surface, which until now have remained difficult to recognize. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Sadna Budhu et al.

Cell reports, 34(2), 108620-108620 (2021-01-14)

Phosphatidylserine (PS) is exposed on the surface of apoptotic cells and is known to promote immunosuppressive signals in the tumor microenvironment (TME). Antibodies that block PS interaction with its receptors have been shown to repolarize the TME into a proinflammatory

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