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Anti-Nitrotyrosine Antibody, clone 1A6, Alexa Fluor™ 488 conjugate
clone 1A6, Upstate®, from mouse
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biological source
mouse
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
1A6, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable
western blot: suitable
isotype
IgG2bκ
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NOS1(4842)
Application
This Anti-Nitrotyrosine Antibody, clone 1A6, Alexa Fluor 488 conjugate is validated for use in FC, WB for the detection of Nitrotyrosine.
Quality
Routinely evaluated by immunoblot.
Physical form
Protein G Purified mouse monoclonal in a solution of 1% BSA, PBS, 0.05% Tween, and 0.05% sodium azide as a preservative.
Storage and Stability
Stable for 1 year at 2–8°C from date of shipment in undiluted aliquots. Upon receipt, and prior to removing the cap, centrifuge the vial to remove any material in the cap. DO NOT FREEZE. Store at 2–8°C in aliquots, in the dark. Avoid repeated warming/cooling cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Biological selectivity and functional aspects of protein tyrosine nitration.
Biochemical and biophysical research communications, 305, 776-783 (2003)
Relevance of protein nitration in brain injury: a key pathophysiological mechanism in neurodegenerative, autoimmune, or inflammatory CNS diseases and stroke.
Amino Acids, 25, 427-436 (2003)
Compartmentalized nitrosation and nitration in mitochondria.
Antioxidants & Redox Signaling, 5, 349-354 (2003)
The Journal of cell biology, 193(5), 867-884 (2011-06-01)
Atherosclerosis is readily observed in regions of blood vessels where disturbed blood flow (d-flow) is known to occur. A positive correlation between protein kinase C ζ (PKCζ) activation and d-flow has been reported, but the exact role of d-flow-mediated PKCζ
The Journal of neuroscience : the official journal of the Society for Neuroscience, 17(18), 6908-6917 (1997-09-15)
We investigated the role of neuronal (type I) nitric oxide synthase (nNOS) in NMDA-mediated excitotoxicity in wild-type (SV129 and C57BL/6J) and type I NOS knock-out (nNOS-/-) mice and examined its relationship to apoptosis. Excitotoxic lesions were produced by intrastriatal stereotactic
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