Skip to Content
Merck
CN
All Photos(1)

Documents

16-202A

Sigma-Aldrich

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate

clone JBW301, Upstate®, from mouse

Synonym(s):

H2AXS139P, Histone H2A.X (phospho S139), H2A histone family, member X, H2AX histone

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

JBW301, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

NM_002105.2

UniProt accession no.

P16104

shipped in

dry ice

target post-translational modification

phosphorylation (pSer139)

Gene Information

human ... H2AX(3014)

Related Categories

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specificity

Broad species cross-reactivity is expected based on conservation of sequence homology.
Recognizes Histone H2A.X phosphorylated at Ser139, MW 15 kDa.

Immunogen

Epitope: Ser139
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse. Clone JBW301.

Application

Immunocytochemistry: 2-4 μg/mL of a previous lot detected phospho-histone H2A.X in etoposide-treated HeLa cells fixed with 95% ethanol/5% acetic acid (10 minutes), followed by 1% formaldehyde, 0.25% Triton™ X-100 in TBS (5 min.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate (mouse monoclonal antibody) validated in FC, ICC to detect phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone.

Quality

Evaluated by Flow Cytometry to detect Log phase Jurkat cells treated with staurosporine.

Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.

Target description

15 kDa

Physical form

Protein G Purified
Purified FITC-conjugated mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide. Frozen solution.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
Staurosporine-treated Jurkat cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans.
Ismail, IH; Wadhra, TI; Hammarsten, O
Nucleic Acids Research null
Lower phosphorylation of p38 MAPK blocks the oxidative stress-induced senescence in myeloid leukemic CD34(+)CD38 (-) cells.
Yin Xiao,Ping Zou,Jie Wang,Hui Song,Jing Zou,Lingbo Liu
Journal of Huazhong University of Science and Technology: Medical Sciences (Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban) null
Deqiang Ding et al.
Nature communications, 8(1), 819-819 (2017-10-12)
Piwi-interacting RNAs are small regulatory RNAs with key roles in transposon silencing and regulation of gametogenesis. The production of mature piwi-interacting RNAs requires a critical step of trimming piwi-interacting RNA intermediates to achieve optimally sized piwi-interacting RNAs. The poly(A)-specific ribonuclease
Uros Midic et al.
Molecular reproduction and development, 85(7), 635-648 (2018-06-15)
Structural maintenance of chromosome flexible domain containing 1 (Smchd1) is a chromatin regulatory gene for which mutations are associated with facioscapulohumeral muscular dystrophy and arhinia. The contribution of oocyte- and zygote-expressed SMCHD1 to early development was examined in mice (
David W Schoppy et al.
The Journal of clinical investigation, 122(1), 241-252 (2011-12-03)
Oncogenic Ras and p53 loss-of-function mutations are common in many advanced sporadic malignancies and together predict a limited responsiveness to conventional chemotherapy. Notably, studies in cultured cells have indicated that each of these genetic alterations creates a selective sensitivity to
View All Related Papers

Articles

Flow Cytometry Troubleshooting Guide

Troubleshooting guide offers solutions for common flow cytometry problems, ensuring improved analysis performance.

Flow Cytometry Dye Selection Tips

Flow cytometry dye selection tips match fluorophores to flow cytometer configurations, enhancing panel performance.

Protocols

Flow Cytometry Guide

Explore our flow cytometry guide to uncover flow cytometry basics, traditional flow cytometer components, key flow cytometry protocol steps, and proper controls.

Key Steps in Flow Cytometry Protocols

Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service