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Key Documents

16-202A

Sigma-Aldrich

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate

clone JBW301, Upstate®, from mouse

Synonym(s):

H2AXS139P, Histone H2A.X (phospho S139), H2A histone family, member X, H2AX histone

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100 μG
CN¥7,918.28

CN¥7,918.28

Estimated to ship onMay 13, 2025Details

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100 μG
CN¥7,918.28

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥7,918.28

Estimated to ship onMay 13, 2025Details

Sign In for Best Price
Request a Bulk Order

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

JBW301, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG1

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This Item
056361619305636AF488
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate clone JBW301, Upstate®, from mouse

16202A

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 clone JBW301, Upstate®, from mouse

05636

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate clone JBW301, Upstate®, from mouse

16193

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate

Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor™ 488 Conjugate Antibody clone JBW301, from mouse, ALEXA FLUOR™ 488

05636AF488

Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor™ 488 Conjugate Antibody

clone

JBW301, monoclonal

clone

JBW301, monoclonal

clone

JBW301, monoclonal

clone

JBW301, monoclonal

Gene Information

human ... H2AX(3014)

Gene Information

-

Gene Information

human ... H2AX(3014)

Gene Information

human ... H2AX(3014)

conjugate

FITC conjugate

conjugate

-

conjugate

biotin conjugate

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

species reactivity

human

species reactivity

vertebrates

species reactivity

human

species reactivity

human

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specificity

Broad species cross-reactivity is expected based on conservation of sequence homology.
Recognizes Histone H2A.X phosphorylated at Ser139, MW 15 kDa.

Immunogen

Epitope: Ser139
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse. Clone JBW301.

Application

Immunocytochemistry: 2-4 μg/mL of a previous lot detected phospho-histone H2A.X in etoposide-treated HeLa cells fixed with 95% ethanol/5% acetic acid (10 minutes), followed by 1% formaldehyde, 0.25% Triton™ X-100 in TBS (5 min.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate (mouse monoclonal antibody) validated in FC, ICC to detect phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone.

Quality

Evaluated by Flow Cytometry to detect Log phase Jurkat cells treated with staurosporine.

Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.

Target description

15 kDa

Physical form

Protein G Purified
Purified FITC-conjugated mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide. Frozen solution.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
Staurosporine-treated Jurkat cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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