16-103
Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate
clone 4G10®, Upstate®, from mouse
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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
4G10®, monoclonal
species reactivity
human
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2bκ
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr)
Gene Information
human ... PID1(55022)
General description
Anti-phosphotyrosine monoclonal antibody, clone 4G10 (cat.# 05-321), cross-linked to biotin.
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.
Description
Anti-phosphotyrosine monoclonal antibody, clone 4G10 (cat. # 05-321), cross-linked to biotin.
Description
Anti-phosphotyrosine monoclonal antibody, clone 4G10 (cat. # 05-321), cross-linked to biotin.
Specificity
Expected to cross-react with rat and mouse.
Recognizes tyrosine-phosphorylated proteins from all species.
Immunogen
Phosphotyramine-KLH.
Application
Anti-Phosphotyrosine Antibody, clone 4G10, Biotin Conjugate detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is published in peer reviewed journals and validated for use in IC, IP, WB.
Immunoprecipitation:
4 μg of a previous lot, used in conjunction with Streptavidin, agarose conjugate (Catalog # 16-126), immunoprecipitated phosphotyrosine containing proteins from a lysate of EGFstimulated A431 cells.
Note: To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
Immunocytochemistry:
5-10 μg/mL of a previous lot gave positive immunostaining of EGF-stimulated A431 cells that had been fixed with ethanol:acetic acid [1:1].
Application Notes
For use in applications in which a biotin conjugate is advantageous, such as WB and IC.
4 μg of a previous lot, used in conjunction with Streptavidin, agarose conjugate (Catalog # 16-126), immunoprecipitated phosphotyrosine containing proteins from a lysate of EGFstimulated A431 cells.
Note: To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
Immunocytochemistry:
5-10 μg/mL of a previous lot gave positive immunostaining of EGF-stimulated A431 cells that had been fixed with ethanol:acetic acid [1:1].
Application Notes
For use in applications in which a biotin conjugate is advantageous, such as WB and IC.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Quality
Routinely evaluated by Western Blot on EGF treated A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Tyrosine phosphorylated proteins on 10 μg of EGF treated A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Tyrosine phosphorylated proteins on 10 μg of EGF treated A431 lysates.
Target description
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physical form
PBS containing 0.05% sodium azide.
Protein G Purified
Storage and Stability
9 months at 2-8°C from date of shipment. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Analysis Note
Control
Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells
Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Imran H Khan et al.
Molecular & cellular proteomics : MCP, 5(4), 758-768 (2005-12-22)
Phosphorylation analysis of signaling proteins is key for examining intracellular signaling pathways. Conventional biochemical approaches, e.g. immunoprecipitation, Western blot, and ELISA, have played a major role in elucidation of individual signaling events. However, these methods are laborious, time-consuming, and difficult
Sandra Odom et al.
The Journal of experimental medicine, 199(11), 1491-1502 (2004-06-03)
A role for Lyn kinase as a positive regulator of immunoglobulin (Ig)E-dependent allergy has long been accepted. Contrary to this belief, Lyn kinase was found to have an important role as a negative regulator of the allergic response. This became
H Nishizumi et al.
The Journal of experimental medicine, 187(8), 1343-1348 (1998-05-23)
B cells from young lyn-/- mice are hyperresponsive to anti-IgM-induced proliferation, suggesting involvement of Lyn in negative regulation of B cell antigen receptor (BCR)-mediated signaling. Here we show that tyrosine phosphorylation of FcgammaRIIB and CD22 coreceptors, which are important for
Eivind F Finne et al.
The Biochemical journal, 379(Pt 1), 39-46 (2003-12-25)
Ephrins are ligands for the Eph receptor tyrosine kinases, which play important roles in patterning nervous and vascular systems. Ephrin-A1 is a glycosylphosphatidylinositol-anchored ligand that binds to the EphA receptor tyrosine kinases. In the present study, we have identified a
Replication stress induces micronuclei comprising of aggregated DNA double-strand breaks.
Xu B, Sun Z, Liu Z, Guo H, Liu Q, Jiang H, Zou Y, Gong Y, Tischfield JA, Shao C
Testing null
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