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1.51921

Supelco

Purospher® STAR Phenyl (5 µm) HPLC Columns

L × I.D. 250 mm x 4.6 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

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Synonym(s):
Purospher® STAR Phenyl (5µm) LiChroCART® 250-4.6
UNSPSC Code:
41115700
NACRES:
SB.52

product name

Purospher® STAR Phenyl (5 µm), L × I.D. 250 mm x 4.6 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

material

stainless steel column

Quality Level

100

Agency

suitable for USP L11

product line

Purospher®

feature

endcapped: no

packaging

1 ea of

extent of labeling

12.5% Carbon loading

parameter

250 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

25 cm × 4.6 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

Phenyl bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase

storage temp.

no temp limit

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General description

Purospher® STAR Phenyl, 5 µm UHPLC columns are the best alternative to RP-8 or RP-18 columns for the efficient separation of aromatic compounds, and compounds containing aromatic groups. Purospher® STAR Phenyl can retain analytes via several different mechanisms, including ο-ο interactions between the overlap of the delocalized electrons on the analyte and the stationary phase phenyl group, and via partitioning between the mobile phase and the hydrophobic aryl-alkyl phase. Our Purospher® STAR Phenyl columns are based on high purity silica particles, which provide symmetrical peaks for basic compounds, as well as high stability and excellent reproducibility. Furthermore, hydrophobic compounds elute much faster with Purospher® STAR Phenyl columns than with C-18 columns.Purospher® STAR Phenyl, 5 µm LiChroCART® 250-4.6 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001. LiChroCART® cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.For higher pressure stability requirements Purospher® STAR Phenyl, 5 µm Hibar® RT 250-4.6 HPLC columns are available part number 1.51918.0001. The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for HPLC high-resolution separation of complex samples.Item 1.51921.1003 is a Validation kit containing 3 Purospher® STAR Phenyl, 5 µm LiChroCART® 250-4.6 HPLC columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of antibiotics,aromatic amines, flavonoids, ketones,nucleosides, PAHs, peptides, preservatives, steroids, metabolized steroids, and sweeteners.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Enhanced selectivity for aromatic compounds
  • Suitable for use with highly aqueous mobile phases
  • Good suitability for LC-MS applications

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Regulatory Information

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