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LiChrospher® 100 NH2 (5 µm) HPLC Columns

L x I.D. 250 mm x 4 mm, LiChroCART® HPLC cartridge, manuCART™ holder required for use

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Synonym(s):
TM=["LiChrospher"] Amino TM=["LICHROCART"] cartridge for HPLC
UNSPSC Code:
41115709
NACRES:
SB.52

product name

LiChrospher® 100 NH2 (5 µm) LiChroCART® 250-4, suitable for HPLC

material

stainless steel column

Quality Level

100

Agency

suitable for USP L8

product line

LiChrospher®

feature

endcapped: no

extent of labeling

4.6% Carbon loading

parameter

250 bar pressure
60 °C max. temp.

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (Low)

column L × I.D.

25 cm × 4 mm

surface area

350 m2/g

matrix

fully porous particle

matrix active group

NH2 bonding phase

particle size

5 μm

pore size

100 Å pore size

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

storage temp.

no temp limit

Related Categories

General description

HPLC cartridge
LiChrospher® NH2 posses polar and hydrophobic properties and can be used for normal phase, reversed phase and ion exchange chromatography. Typical applications are the separations of carbohydrates by reversed phase chromatography or the separsation of nucleotides by ion exhange chromatography. This sorbent is packed into a LiChroCART 250-4 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).

Application


  • α-Naphtholbenzein based heterocyclic sensor for detection of Cu(II) ions.: This study introduces a novel application of 1-Naphtholbenzein as a fluorescent sensor for the detection of copper ions in various environmental samples. The heterocyclic design enhances the sensitivity and selectivity, making it valuable for real-time monitoring and ecological studies. This innovation not only advances the field of chemical sensors but also contributes to environmental chemistry by providing a tool for the detection of potentially toxic metal ions (Saini et al., 2024).


Analysis Note

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 45000
Symmetry (2-Nitrotoluene): 0.8 - 1.7
Capacity factor (2-Nitrotoluene): 0.8 - 1.8

Legal Information

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

Certificates of Analysis (COA)

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