Skip to Content
Merck
CN
All Photos(1)

Documents

1.50661

Supelco

SeQuant® ZIC®-cHILIC 100Å (3 µm) HPLC Columns

L x I.D. 50 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

Synonym(s):

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

SeQuant® ZIC®-cHILIC, 3µm, 100Å, 150 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC

material

PEEK-lined stainless steel column

Quality Level

100

Agency

suitable for USP L114

product line

SeQuant®

feature

endcapped: no

parameter

500 bar pressure
70 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

15 cm × 4.6 mm

matrix

fully porous particle

matrix active group

ZIC-HILIC bonding phase

particle size

3 μm

pore size

100 Å pore size

operating pH

2-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

Related Categories

General description

SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.The column dimension of 150 mm length x 4.6 mm ID is the best choice for the separation of polar compounds using a standard HPLC Instrument.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

Pressure: ≤ 50 bar
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service