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Supelco

SeQuant® ZIC®-HILIC 200Å (3.5 µm) HPLC Columns

L × I.D. 150 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

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Synonym(s):
Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
UNSPSC Code:
41115709

product name

SeQuant® ZIC-HILIC™ 3.5µm,200Å 150 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

material

PEEK-lined stainless steel column

Quality Level

100

Agency

suitable for USP L114

product line

SeQuant®

feature

endcapped: no

parameter

550 bar pressure
70 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

15 cm × 2.1 mm

matrix

fully porous particle

matrix active group

ZIC-HILIC bonding phase

particle size

3.5 μm

pore size

200 Å pore size

operating pH

2-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

Related Categories

General description

SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 150 mm length x 2.1 mm ID is the optimal choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Application


  • Click postsynthesis of microporous organic network@silica composites: Demonstrates the application of SeQuant ZIC-HILI columns in mixed-mode chromatography, particularly in the synthesis of composites that facilitate both reversed-phase and hydrophilic interaction chromatography. This approach is crucial for advanced pharmaceutical compound analysis and biomolecule separation technology (Sun HF et al., 2023).

  • Evaluation of Hold-Up Volume Determination Methods and Markers in Hydrophilic Interaction Liquid Chromatography: Focuses on refining the precision in volume determinations using ZIC-HILIC columns, enhancing method development for pharmaceuticals and providing a basis for high-resolution mass spectrometry applications (Redon L et al., 2023).

  • Determination of the polyphenolic content of berry juices: Utilizes SeQuant ZIC-HILIC technology for comprehensive two-dimensional liquid chromatography, essential for complex sample analysis in food safety and quality assessments (Arena K et al., 2023).

  • Simple and sensitive determination of sulfites in Chinese herbal teas: Highlights the robustness of SeQuant ZIC-HILIC in ultra-high-performance liquid chromatography tandem mass spectrometry, ensuring the safety and compliance of herbal products with stringent health standards (Yang L et al., 2022).

Analysis Note

Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.40

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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Determination of Ethyl Glucuronide and Ethyl Sulfate in Urine using LC-MS/MS

This study is aimed at developing and validating an LC-ESI-ion trap-MS/MS method for identification and quantification of ethyl glucuronide (EtG) and ethyl sulfate (EtS) as ethanol biomarkers for urine analysis.

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