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Supelco

Purospher® STAR RP-18 endcapped (3 µm) HPLC Columns

L × I.D. 50 mm x 3 mm Hibar® RT HPLC Column

Synonym(s):

Purospher® STAR RP-18 endcapped (3µm) Hibar® RT 50-3

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Purospher® STAR RP-18 endcapped (3 µm), L × I.D. 50 mm x 3 mm Hibar® RT HPLC column

material

stainless steel column

Quality Level

100

Agency

suitable for ASTM® 7968
suitable for DIN 15968-2010
suitable for EPA 1633
suitable for EPA 38407-42
suitable for EPA 533
suitable for EPA 537.1
suitable for EPA 8327
suitable for EPA ACB B21-02
suitable for EPA ACB B23-05b
suitable for GB 5009.253-2016
suitable for ISO 21675 2019
suitable for ISO 25101
suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

400 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

5 cm × 3 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

3 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase
supercritical fluid (SFC)

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General description

Purospher® STAR RP-18 endcapped, 3 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher® STAR RP-18 endcapped, 3 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher® STAR RP-18 endcapped, 3 µm Hibar® RT 50-3 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 50 mm length x 3 mm I.D. is the optimal choice for fast HPLC separation of complex samples at lower flow rate, allowing significant solvent savings and improved sensitivity. A 3 mm I.D. column enables a more sustainable separation in comparison to 4 or 4.6 mm I.D. column due to the use of less solvent for the chromatographic separation.The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART® 4-4 part number 1.50250.0001. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART® cartridges, part number 1.51487.0001 can be used.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Legal Information

ASTM is a registered trademark of American Society for Testing and Materials
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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