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Supelco

Purospher®STAR RP-8 endcapped (5 µm) HPLC Columns

L × I.D. 250 mm x 4 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

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Synonym(s):
Purospher® STAR RP-8 endcapped (5 µm) LiChroCART® 250-4
UNSPSC Code:
41115709
NACRES:
SB.52

product name

Purospher® STAR RP-8 endcapped (5 µm), L × I.D. 250 mm x 4 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

material

stainless steel column

Quality Level

100

Agency

suitable for USP L7

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

11.2% Carbon loading

parameter

250 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

25 cm × 4 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C8 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase

storage temp.

no temp limit

Related Categories

General description

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher® STAR RP-18 endcapped, 5 µm LiChroCART® 250-4 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001. The manuCART™ can be used to hold one cartridge column with or without direct integration of a 4-4 mm guard column. The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART® 4-4 part number 1.50250.0001. LiChroCART® cartridge columns mounted with a manuCART™ cartridge column holder are hand-tight only and have therefore a maximum pressure stability of 250 bar. For higher pressure stability requirements Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 250-4 HPLC columns are available part number 1.50037.0001.The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for high resolution HPLC separation of complex samples.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, amino acids, esters, fatty acids, ketones, nitrosamines, nucleosides, PCBs, peptides, phenols, preservatives, steroids, and metabolized steroids.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 125 bar
Theoretical Plates (N/m) (Toluene): ≥ 85000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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