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Supelco

Purospher® STAR NH2 (5 µm) HPLC Columns

L × I.D. 125 mm x 4 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

Synonym(s):

Purospher STAR NH2 (5 µm) LiChroCART® 125-4

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Purospher® STAR NH2 (5 µm), L × I.D. 125 mm x 4 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

material

stainless steel column

Quality Level

100

Agency

suitable for USP L8

product line

Purospher®

feature

endcapped: no

manufacturer/tradename

Purospher®

packaging

1 ea of

extent of labeling

3.5% Carbon loading

parameter

250 bar pressure

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

12.5 cm × 4 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

NH2 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

storage temp.

no temp limit

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General description

Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm LiChroCART® 125-4 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART® 4-4 part number 1.50267.0001 LiChroCART® cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.The column dimension of 125 mm length x 4 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%)
  • Low silanol activity
  • Reproducible results from run-to-run and batch-to-batch

Analysis Note

Pressure: ≤ 30 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0

Legal Information

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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