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Supelco

Purospher® STAR RP-18 endcapped (3 µm) HPLC Columns

L × I.D. 30 mm x 4 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

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Synonym(s):
Purospher® STAR RP-18 endcapped (3 µm) LiChroCART® 30-4
UNSPSC Code:
41115709
NACRES:
SB.52

product name

Purospher® STAR RP-18 endcapped (3 µm), L × I.D. 30 mm x 4 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

250 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

3 cm × 4 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

3 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase

storage temp.

no temp limit

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General description

Purospher® STAR RP-18 endcapped, 3 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher® STAR RP-18 endcapped, 3 µm LiChroCART® 30-4 is a cartridge column which requires a manuCART™ 30 mm cartridge column holder part number 1.50227.0001. LiChroCART® cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and has therefore a maximum pressure stability of 250 bar. The column dimension of 30 mm length x 4 mm I.D. is the optimal choice for very fast HPLC separations. Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, amino acids, esters, fatty acids, ketones, nitrosamines, nucleosides, PCBs, peptides, phenols, preservatives, steroids, and metabolized steroids.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 30 bar
Theoretical Plates (N/m) (1,2-Benzanthracene): ≥ 120000
Symmetry (1,2-Benzanthracene): 0.8 - 1.4
Capacity factor (1,2-Benzanthracene): ≥ 7.0

Legal Information

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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