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1.50035

Supelco

Purospher®STAR RP-8 endcapped (5 µm) HPLC Columns

L × I.D. 250 mm x 4 mm Hibar® RT HPLC Column

Synonym(s):

Purospher® STAR RP-8 endcapped (5µm) Hibar® RT 250-4

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Purospher® STAR RP-8 endcapped (5 µm), L × I.D. 250 mm x 4 mm Hibar® RT HPLC column

material

stainless steel column

Quality Level

100

Agency

suitable for USP L7

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

11.2% Carbon loading

parameter

400 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

25 cm × 4 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C8 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase
supercritical fluid (SFC)

storage temp.

no temp limit

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General description

Purospher® STAR RP-8 endcapped, 5 µm HPLC columns allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range. Purospher® STAR RP-8 endcapped, 5 µm Hibar® RT 250-4 is a ready to use UHPLC column suitable for connection to any HPLC system.The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for the HPLC separation of complex samples.Item 1.50035.1003 is a Validation kit containing 3 Purospher® STAR RP-8 endcapped, 5 µm Hibar® RT 250-4 columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, amino acids, esters, fatty acids, ketones, nitrosamines, nucleosides, PCBs, peptides, phenols, preservatives, steroids, and metabolized steroids.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Outstanding pH stability from pH 1.5 to 10.5
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 125 bar
Theoretical Plates (N/m) (Toluene): ≥ 85000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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