CLK1, active

CLK1 is a dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.

Product Source: N-terminal 6His-tagged, recombinant, human CLK1, amino acids 130-end expressed by baculovirus in Sf21 insect cells

Target Sub-Family: CMGC

Also available in 250μg size as catalog # 14-920M and in bulk as catalog # 14-920-K.

Routinely evaluated by phosphorylation of peptide ERMRPRKRQGSVRRRV

50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol

Purified using immobilized metal affinity chromatography.

6 months at -70°C

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

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