14-777-M
Plk1 Protein, active, 10 µg
Active, N-terminal 6His tagged, recombinant, human Plk1. For use in Kinase Assays.
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UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41
Recommended Products
biological source
human
Quality Level
recombinant
expressed in baculovirus infected Sf21 cells
mol wt
Mw 69.3 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
General description
N-terminal 6His tagged, recombinant, human Plk1.
Product Source: Expressed by baculovirus in Sf21 insect cells.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Biochem/physiol Actions
Protein Target: Plk1
Target Sub-Family: Misc
Packaging
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-777M when ordering the 250 µg size.
Physical form
50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose
Storage and Stability
Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Shrividya Sana et al.
Life science alliance, 1(6), e201800223-e201800223 (2018-11-21)
Proper orientation of the mitotic spindle defines the correct division plane and is essential for accurate cell division and development. In metazoans, an evolutionarily conserved complex comprising of NuMA/LGN/Gαi regulates proper orientation of the mitotic spindle by orchestrating cortical dynein
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