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14-770-M

Sigma-Aldrich

mTOR (1362-end) Protein, active, 10 µg

Active, N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end, for use in Kinase Assays.

Synonym(s):

FRAP1

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CN¥964.58
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CN¥4,519.63

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10 G
CN¥964.58
50 G
CN¥4,519.63

About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

CN¥964.58

Please contact Customer Service for Availability
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Request a Bulk Order

biological source

human

Quality Level

100

recombinant

expressed in baculovirus infected Sf21 cells

form

liquid

specific activity

377 U/mg

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

solubility

water: soluble

NCBI accession no.

NM_004958.2

UniProt accession no.

P42345

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mTOR (1362-end) Protein, active, 10 µg Active, N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end, for use in Kinase Assays.

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FGFR2 Protein, active, 10 µg Active, N-terminal, His6-tagged, recombinant, human FGFR2, amino acids 456-770, for use in Kinase Assays.

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FGFR2 Protein, active, 10 µg

biological source

human

biological source

-

biological source

human

biological source

human

technique(s)

activity assay: suitable (kinase)

technique(s)

-

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

form

liquid

form

buffered aqueous solution

form

-

form

-

storage temp.

−70°C

storage temp.

−20°C

storage temp.

-

storage temp.

-

UniProt accession no.

P42345

UniProt accession no.

P42345

UniProt accession no.

P22455

UniProt accession no.

P21802

General description

Research area: Cell Signaling

Protein Target: mTOR (FRAP1)

Target Sub-Family: Lipid/Atypical

N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end

The mechanistic target of rapamycin (mTOR) protein is a serine/threonine protein kinase belonging to PI3K-related kinase (PIKK) family, expressed throughout the body.[1][2]

Application

The active mTOR (1362-end) protein, 10 µg, has been used in the mTOR substrate phosphorylation assay to validate the biochemical and pharmacological profile of a novel mTOR-KI for targeting mTOR signaling pathways in vitro and in vivo.[3]

Biochem/physiol Actions

Mechanistic target of rapamycin (mTOR) plays a pivotal role in regulating various essential cellular processes, ranging from protein synthesis to autophagy.[2] Moreover, it influences metabolism, cellular survival, gene transcription, and cytoskeletal components. It gets activated in response to growth factors, nutrients, mitogens, and hormones.[1]Downregulation of mTOR signaling is associated with the advancement of cancer and diabetes, and aging process.[2]

Packaging

Also available in 100μg size, please inquire for pricing and availability.

Unit Definition

Specific Activity: where one unit of mTOR activity is defined as 1 nmole of phosphate incorporated into 2mg/ml mTOR substrate per minute at 30°C with a final ATP concentration of 100µM.

Physical form

16µg of enzyme in 100µl of 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 300µg/ml FLAGTM peptide, 20% v/v glycerol

Storage and Stability

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

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    mTOR Signaling in Growth, Metabolism, and Disease
    Saxton RA and Sabatini DM
    Cells, 168(6), 960?976- 960?976 (2017)
    mTOR: Exploring a New Potential Therapeutic Target for Stroke
    Castellanos M, et al.
    Molecules to Medicine with mTOR, 105-122 (2016)
    Jianchang Qian et al.
    Oncotarget, 7(41), 67071-67086 (2016-08-27)
    The mechanistic target of rapamycin (mTOR) is a rational target for cancer treatment. While the mTORC1-selective rapalogs have shown significant benefits in the clinic, antitumor response may be further improved by inhibiting both mTORC1 and mTORC2. Herein, we established target

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