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Merck
CN

14-757

Abl (M351T) Protein, active, 10 µg

Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the M351T mutation. For use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405

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Product Name

Abl (M351T) Protein, active, 10 µg, Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the M351T mutation. For use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 121 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

UniProt accession no.

P00519
U07563

shipped in

dry ice

Quality Level

100

Analysis Note

Routinely evaluated by phosphorylation of Abltide.

Biochem/physiol Actions

Protein Target: Abl
Target Sub-Family: TK

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the M351T mutation. Expressed by baculovirus in Sf21 insect cells.
The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the M351T mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125).

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250μg size as catalog # 14-757M - Call for pricing and availability.

Physical form

50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.
Ni2+/NTA-agarose

Preparation Note

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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