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Merck
CN

14-477-M

Cdk5/p35 Protein, active, 10 µg

Active complex of recombinant full length human Cdk5 containing & N-terminal His6-tag & recombinant full-length human p35 containing an N-terminal GST-tag, for use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26
eCl@ss:
32160405

Key Documents

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Product Name

Cdk5/p35 Protein, active, 10 µg, Active complex of recombinant full length human Cdk5 containing & N-terminal His6-tag & recombinant full-length human p35 containing an N-terminal GST-tag, for use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 34 kDa (cdk5)
Mw 61 kDa (p35 with GST-tag)

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_004935.2

UniProt accession no.

Q00535

Quality Level

100

Analysis Note

routinely evaluated by phosphorylation of Histone H1

Biochem/physiol Actions

Protein Target: CDK5/p35
Target Sub-Family: CMGC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Complex of recombinant fulllength human cdk5 containing and N-terminal His6-tag and recombinant full-length human p35 containing an N-terminal GST-tag
Product Source: Both are expressed by baculovirus in Sf21 insect cells

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250μg size as catalog # 14-477M - Call for pricing and availability.

Physical form

Ni-NTA agarose

Preparation Note

1 year at -70°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ward G Walkup et al.
The Journal of biological chemistry, 290(8), 4908-4927 (2014-12-24)
synGAP is a neuron-specific Ras and Rap GTPase-activating protein (GAP) found in high concentrations in the postsynaptic density (PSD) fraction from the mammalian forebrain. We have previously shown that, in situ in the PSD fraction or in recombinant form in
Ward G Walkup et al.
eLife, 5 (2016-10-21)
SynGAP is a Ras/Rap GTPase-activating protein (GAP) that is a major constituent of postsynaptic densities (PSDs) from mammalian forebrain. Its α1 isoform binds to all three PDZ (PSD-95, Discs-large, ZO-1) domains of PSD-95, the principal PSD scaffold, and can occupy

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