14-475-M
Cdk2/Cyclin E Protein, active, 10 µg
Active complex of recombinant full length human Cdk2 containing a C-terminal His6-tag & recombinant full-length human Cyclin E containing an N-terminal GST-tag. For use in Kinase Assays.
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UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41
Recommended Products
biological source
human
Quality Level
recombinant
expressed in baculovirus infected Sf21 cells
mol wt
Mw 34 kDa (cdk2)
Mw 74 kDa (cyclin)
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
General description
Research area: APOPTOSIS
Complex of recombinant full length human cdk2 containing a C-terminal His6-tag, and recombinant full-length human cyclin E containing an N-terminal GST-tag. Product Source: Both are expressed by baculovirus in Sf21 insect cells The Cyclin E genes (CCNE1) gene located at 19q12 region in humans is responsible for encoding full-length protein of 410 amino acids.
Complex of recombinant full length human cdk2 containing a C-terminal His6-tag, and recombinant full-length human cyclin E containing an N-terminal GST-tag. Product Source: Both are expressed by baculovirus in Sf21 insect cells The Cyclin E genes (CCNE1) gene located at 19q12 region in humans is responsible for encoding full-length protein of 410 amino acids.
Biochem/physiol Actions
Protein Target: CDK2/cyclinE
Target Sub-Family: CMGC
The Cyclin E1 protein binds to and activates Cyclin-dependent kinase 2 (CDK2), forming an active complex crucial for the initiation and progression of the S phase. Cyclin E/CDK2 complex primarily regulates cell cycle advancement and DNA replication by phosphorylating specific substrates. Elevated Cyclin E protein levels and heightened CDK2 kinase activity are both individually linked to unfavorable prognosis, decreased survival, and resistance to therapy in cancer patients. Overactivation of the Cyclin E/CDK2 complex directly disrupts DNA replication through multiple mechanisms, resulting in DNA double strand breaks (DSBs) and genomic instability. Targeting the oncogenic Cyclin E/CDK2 complex has emerged as a potential therapeutic approach against cancer.
Target Sub-Family: CMGC
The Cyclin E1 protein binds to and activates Cyclin-dependent kinase 2 (CDK2), forming an active complex crucial for the initiation and progression of the S phase. Cyclin E/CDK2 complex primarily regulates cell cycle advancement and DNA replication by phosphorylating specific substrates. Elevated Cyclin E protein levels and heightened CDK2 kinase activity are both individually linked to unfavorable prognosis, decreased survival, and resistance to therapy in cancer patients. Overactivation of the Cyclin E/CDK2 complex directly disrupts DNA replication through multiple mechanisms, resulting in DNA double strand breaks (DSBs) and genomic instability. Targeting the oncogenic Cyclin E/CDK2 complex has emerged as a potential therapeutic approach against cancer.
Packaging
Also available in 250μg size as catalog # 14-475M - Call for pricing and availability.
Quality
routinely evaluated by phosphorylation of histone H1 by cdk2/cyclin E
Physical form
Ni-NTA agarose
Storage and Stability
1year at -70C
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Cyclin E/CDK2: DNA Replication, Replication Stress and Genomic Instability
Fagundes R and Teixeira LK
Frontiers in Cell and Developmental Biology, 9, 774845-774845 (2021)
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