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14-442-M

Sigma-Aldrich

Lck Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant full-length human Lck, for use in Kinase Assays.

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UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

100

recombinant

expressed in Sf21 cells

product line

Upstate®

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

mol wt

Mw 59 kDa

purified by

(Ni2+/NTA-agarose)

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

accession no.

 (FUNCTION: SwissProt: P06239 # Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyp)
NM_001042771.1

NCBI accession no.

NM_001042771.1
NM_005356.3

UniProt accession no.

P06239

General description

N-terminal His6-tagged, recombinant full-length human Lck

Biochem/physiol Actions

Protein Target: Lck
Target Sub-Family: TK

Packaging

Also available in 10 μg size and in bulk
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-442M when ordering the 250 µg size.

Quality

routinely evaluated by phosphorylation of Src substrate peptide

Target description

Lck

Physical form

Ni2+/NTA-agarose

Storage and Stability

6 months at -70C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Coordination of T cell activation and migration through formation of the immunological synapse.
Dustin, Michael L
Annals of the New York Academy of Sciences, 987, 51-59 (2003)
TCR trafficking in resting and stimulated T cells.
Geisler, Carsten
Critical Reviews in Immunology, 24, 67-86 (2004)
Emil H Palacios et al.
Oncogene, 23(48), 7990-8000 (2004-10-19)
The function of the Src-family kinases (SFKs) Lck and Fyn in T cells has been intensively studied over the past 15 years. Animal models and cell line studies both indicate a critical role for Lck and Fyn in proximal T-cell
Rose Zamoyska et al.
Immunological reviews, 191, 107-118 (2003-03-05)
The src-family kinases p56lck (Lck) and p59fyn (Fyn) are expressed in T cells and are among the first signaling molecules to be activated downstream of the T cell receptor (TCR). Evidence is emerging that although closely related, these signaling molecules

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