Skip to Content
Merck
CN

129935

Actinomycin D, 7-Amino-

7-Aminoactinomycin D, like its parent molecule, actinomycin D, is a DNA intercalator and has antibacterial properties. Exhibits growth-inhibitory activity against certain leukemias and sarcomas.

Synonym(s):

Actinomycin D, 7-Amino-, 7-AAD, 7-Amino-AMD, 7-Aminoactinomycin C1, 7-Aminodactinomycin

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C62H87N13O16
CAS Number:
7240-37-1
Molecular Weight:
1270.43
UNSPSC Code:
12352200
MDL number:
MFCD00077295

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Actinomycin D, 7-Amino-, 7-Aminoactinomycin D, like its parent molecule, actinomycin D, is a DNA intercalator and has antibacterial properties. Exhibits growth-inhibitory activity against certain leukemias and sarcomas.

InChI

1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)

InChI key

YXHLJMWYDTXDHS-UHFFFAOYSA-N

assay

≥85% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

red-purple

input

sample type intact cells

solubility

DMSO: 20 mg/mL
ethanol: 5 mg/mL

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Biochem/physiol Actions

Primary Target
leukemias and sarcomas

Disclaimer

Toxicity: Highly Toxic (H)

General description

7-Aminoactinomycin D is a membrane impermeable fluorescent DNA intercalator. Like its parent compound, Actinomycin D (Cat. No. 114666), 7-AAD has antibacterial properties and growth inhibitory activity against certain leukemias and sarcomas. Useful for flow cytometry and fluorescence microscopy. Used for staining nonviable cells and for identifying apoptotic cells. Compatible with FITC and PE cell surface staining. The absorption/emission maxima are as follows: unbound (ex. 500 nm, em. 672 nm) and DNA bound (ex. 555 nm, em. 655 nm). λmax 528 nm ± 2; εmax = 22,700 ± 5,000 M-1 cm-1
7-Aminoactinomycin D, like its parent molecule, actinomycin D, is a DNA intercalator and has antibacterial properties. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. Fluorescent excitation and emission maxima are 500 and 672 nm, respectively. Complexes with DNA and absorbs at 555 nm and emits at 655 nm. Useful in distinguishing early apoptotic cells, which have lost membrane integrity, from apoptotic and live cells. Has been used in DNA analysis and as a probe for chromosome structure and function.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Schmid, I., et al. 1994. J. Immunol. Methods 170, 145.
Berg, R.J., et al. 1993. Carcinogenesis14, 103.
Chen, F.M. 1992. Biochemistry31, 6223.
Schmid, I., et al. 1992. Cytometry13, 204.
Toba, K., et al. 1992. Cytometry13, 60.
Sengupta, S.K., et al. 1975. J. Med. Chem.18, 1175.

Preparation Note

Following reconstitution aliquot, purge with inert gas and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
Further dilute in aqueous buffers just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

Regulatory Information

涉药品监管产品
This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

David W Doo et al.
Therapeutic advances in medical oncology, 12, 1758835920913798-1758835920913798 (2020-04-22)
The Wnt/β-catenin pathway is linked to tumorigenesis in a variety of tumors and promotes T cell exclusion and resistance to checkpoint inhibitors. We sought to determine whether a small molecule inhibitor of this pathway, WNT974, would impair tumor growth, affect
Xiao-Long Ma et al.
Cell death discovery, 7(1), 309-309 (2021-10-25)
Accumulating evidence has shown that circular RNA (circRNA) dysregulation is involved in various types of cancer, including osteosarcoma (OS). Nevertheless, the role and mechanism of circRNAs in OS progression and chemoresistance remain elusive. We found that a novel doxorubicin-induced circular
Huizhen Yang et al.
Cell biology international, 44(8), 1671-1680 (2020-04-14)
To better treat patients with non-small cell lung cancer (NSCLC), the investigations on novel molecules affecting NSCLC progression are of vital importance. Long noncoding RNAs (lncRNAs) are identified as pivotal regulators that can affect the cellular activities of carcinomas. Long
Whitney N Goldsberry et al.
Cancers, 12(3) (2020-03-28)
In ovarian cancer, upregulation of the Wnt/β-catenin pathway leads to chemoresistance and correlates with T cell exclusion from the tumor microenvironment (TME). Our objectives were to validate these findings in an independent cohort of ovarian cancer subjects and determine whether
David W Doo et al.
Therapeutic advances in medical oncology, 12, 1758835920913798-1758835920913798 (2020-04-22)
The Wnt/β-catenin pathway is linked to tumorigenesis in a variety of tumors and promotes T cell exclusion and resistance to checkpoint inhibitors. We sought to determine whether a small molecule inhibitor of this pathway, WNT974, would impair tumor growth, affect
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service